GADD45GIP1 Rekombinanter Antikörper

GADD45GIP1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240947D1

Kat-Nr. : 83823-5-RR

Synonyme

CRIF1, CR6-interacting factor 1, CKII beta-associating protein, CKBBP2, 39S ribosomal protein L59, mitochondrial

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  WB analysis using 83823-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83823-5-RR (GADD45GIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovarian cancer using 83823-5-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83823-5-RR (GADD45GIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovarian cancer using 83823-5-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83823-5-RR (GADD45GIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, Jurkat-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IHC: human ovarian cancer; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83823-5-RR bindet in WB, IHC, ELISA GADD45GIP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GADD45GIP1 fusion protein Ag9299
• Vollständiger Name: growth arrest and DNA-damage-inducible, gamma interacting protein 1
• Berechnetes Molekulargewicht: 222 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC013027
• Gene symbol: GADD45GIP1
• Gene ID (NCBI): 90480
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Growth arrest and DNA-damage-inducible proteins-interacting protein 1(GADD45GIP1), also known as CRIF1, is newly identified de novo components in large subunit of mitoribosome. It is essential for the translation of mitochondrial oxidative phosphorylation (OXPHOS) polypeptides in mammalian mitochondria. The essential role of CRIF1 in mitochondrial synthesis and membrane integration of OXPHOS polypeptides was shown in brain-specific CRIF1-deficient mice, which exhibited profound OXPHOS failure and marked neurodegeneration. (PMID 22819524) In addition, GADD45GIP1 enhances the function of GADD45 in maintaining genome stability, DNA repair and cell cycle regulation by interacting with the GADD45 protein family. It's reported that GADD45GIP1 may be induced by p53, a process that is closely related to the cell's response to DNA damage.(PMID:28599472)