GALM Rekombinanter Antikörper

GALM Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251049A8

Kat-Nr. : 86369-1-RR

Synonyme

Aldose 1-epimerase, BLOCK25, EC:5.1.3.3, IBD1

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  WB analysis using 86369-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86369-1-RR (GALM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 86369-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using GALM antibody (86369-1-RR, Clone: 251049A8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Mausnierengewebe, Rattennierengewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86369-1-RR bindet in WB, IF/ICC, ELISA GALM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GALM fusion protein Ag8842
• Vollständiger Name: galactose mutarotase (aldose 1-epimerase)
• Berechnetes Molekulargewicht: 342 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC019263
• Gene symbol: GALM
• Gene ID (NCBI): 130589
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GALM, also named as Aldose 1-epimerase, belongs to the aldose epimerase family. GALM is a mutarotase that catalyzes the interconversion of beta-D-galactose and alpha-D-galactose during galactose metabolism. GALM is involved in the maintenance of the equilibrium between the beta- and alpha-anomers of galactose, therefore ensuring a sufficient supply of the alpha-anomer for GALK1. GALM is also active on D-glucose, but its preference for galactose is higher than that of glucose. The calculated molecular weight of GALM is 38 kDa.