GALNS Polyklonaler Antikörper

GALNS Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 14933-1-AP

Synonyme

MPS4A, GAS, GALNAC6S, GalN6S, Galactose-6-sulfate sulfatase

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  WB analysis using 14933-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14933-1-AP (GALNS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14933-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14933-1-AP (GALNS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 14933-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14933-1-AP (GALNS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14933-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14933-1-AP (GALNS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Lebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14933-1-AP bindet in WB, IHC, IF, ELISA GALNS und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GALNS fusion protein Ag6746
• Vollständiger Name: galactosamine (N-acetyl)-6-sulfate sulfatase
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC050684
• Gene symbol: GALNS
• Gene ID (NCBI): 2588
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GALNS(N-acetylgalactosamine-6-sulfatase) is also named as chondroitinase and belongs to the sulfatase family. It is one of sulfatases required to degrade glycosaminoglycans (GAGs), keratan sulfate (KS) and chondroitin-6-sulfate (C6S) and the enzyme is a dimer derived from two 60 kDa polypeptides, each of which is processed to 40 kDa and 15 kDa polypeptide subunits linked by disulfide bonds(PMID:15241807). The deduced 522-residue protein is composed of a 26-amino acid N-terminal signal peptide and a mature polypeptide of 496 amino acid residues, including 2 potential asparagine-linked glycosylation sites(PMID:1755850). Defects in GALNS are the cause of mucopolysaccharidosis type 4A (MPS4A), also known as Morquio A syndrome.

Publikationen

Species	Application	Title
human	IHC,IF	Discov Oncol; Identification of galactosamine-(N-acetyl)-6-sulfatase (GALNS) as a novel therapeutic target in progression of nasopharyngeal carcinoma; Authors - Jin Zhang