GALNT14 Polyklonaler Antikörper

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• KD/KO Validated

GALNT14 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 16939-1-AP

Synonyme

GalNac T10, GalNac T14, GALNT14, GALNT15, pp GaNTase 14

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 16939-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16939-1-AP

  WB result of GALNT14 antibody (16939-1-AP; 1:1200; room temperature for 1.5 hours) with wild-type and GALNT14 knockout A549 cells.

• 

  WB analysis of mouse kidney using 16939-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16939-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 16939-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 16939-1-AP (GALNT14 Antibody) at dilution of 1:400 incubated at 4 degree celsius over night.

• 

  IHC staining of human kidney using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary using 16939-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16939-1-AP (GALNT14 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Maushodengewebe, Mauslungengewebe, Mausnierengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hodengewebe, humanes Lebergewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

16939-1-AP bindet in WB, IHC, ELISA GALNT14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GALNT14 fusion protein Ag10470
• Vollständiger Name: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 14 (GalNAc-T14)
• Berechnetes Molekulargewicht: 552 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC010659
• Gene symbol: GALNT14
• Gene ID (NCBI): 79623
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Oncotarget; GalNAc-T14 promotes metastasis through Wnt dependent HOXB9 expression in lung adenocarcinoma.; Authors - Ok-Seon Kwon ; KD Validated
		Mol Cancer Res; BORIS expression in ovarian cancer precursor cells alters the CTCF cistrome and enhances invasiveness through GALNT14.; Authors - Joanna C Hillman
human	IHC	Front Immunol; Vessel state and immune infiltration of the angiogenesis subgroup and construction of a prediction model in osteosarcoma; Authors - Jintao Wu
human	WB	Front Immunol; Molecular characterization of immunogenic cell death indicates prognosis and tumor microenvironment infiltration in osteosarcoma; Authors - Zhongyue Liu ; KD Validated