GALNT2 Polyklonaler Antikörper

GALNT2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17441-1-PBS

Synonyme

pp GaNTase 2, Polypeptide N-acetylgalactosaminyltransferase 2 soluble form, Polypeptide N-acetylgalactosaminyltransferase 2, GalNAc-T2, GalNAc T2

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  WB analysis using 17441-1-AP (same clone as 17441-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17441-1-AP (GALNT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17441-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 17441-1-AP (same clone as 17441-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17441-1-AP (GALNT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17441-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 17441-1-AP (same clone as 17441-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17441-1-AP (GALNT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17441-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 17441-1-AP (same clone as 17441-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GALNT2 antibody (17441-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 17441-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 17441-1-AP (same clone as 17441-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GALNT2 antibody (17441-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 17441-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17441-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GALNT2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GALNT2 fusion protein Ag11523
• Vollständiger Name: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)
• Berechnetes Molekulargewicht: 571 aa, 65 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC041120
• Gene symbol: GALNT2
• Gene ID (NCBI): 2590
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GALNT2, a GalNAc-transferase involved in O-linked glycosylation, modulates the risk of atherogenic dyslipidemia, a specific hallmark of insulin resistance and acts as a positive modulator of insulin signaling in human liver cells by down-regulating ENPP1. GALNT2 gene encodes GalNAc-T2 as a regulator of high-density lipoprotein cholesterol (HDL-C) metabolism (PMID: 31040393, 27508872). Western blot analysis detected GALNT2 at an apparent molecular mass of 64 kDa.