GALNT2 Rekombinanter Antikörper

GALNT2 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240723G10

Kat-Nr. : 83703-2-PBS

Synonyme

240723G10, EC:2.4.1.41, GalNAc T2, GalNAc-T2, Polypeptide N-acetylgalactosaminyltransferase 2

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  WB analysis using 83703-2-RR (same clone as 83703-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83703-2-RR (GALNT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83703-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 83703-2-RR (same clone as 83703-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83703-2-RR (GALNT2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83703-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00666-1

  Cytometric bead array standard curve of MP00666-1, GALNT2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83703-1-PBS. Detection antibody: 83703-2-PBS. Standard: Ag11523. Range: 1.563-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 83703-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83703-2-RR against Human GALNT2 were performed. The affinity constant is 2.04 nM.

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Geprüfte Anwendungen

Produktinformation

83703-2-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA GALNT2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GALNT2 fusion protein Ag11523
• Vollständiger Name: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)
• Berechnetes Molekulargewicht: 571 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC041120
• Gene symbol: GALNT2
• Gene ID (NCBI): 2590
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GALNT2, a GalNAc-transferase involved in O-linked glycosylation, modulates the risk of atherogenic dyslipidemia, a specific hallmark of insulin resistance and acts as a positive modulator of insulin signaling in human liver cells by down-regulating ENPP1. GALNT2 gene encodes GalNAc-T2 as a regulator of high-density lipoprotein cholesterol (HDL-C) metabolism (PMID: 31040393, 27508872). Western blot analysis detected GALNT2 at an apparent molecular mass of 64 kDa.