GALNTL2 Polyklonaler Antikörper

GALNTL2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16900-1-AP

Synonyme

EC:2.4.1.41, GalNAc-T-like protein 2, GALNT15, GALNT7, PIH5

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  WB analysis of HEK-293 using 16900-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16900-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse small intestine using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16900-1-AP (GALNTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 16900-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16900-1-AP (GALNTL2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Lebergewebe
• Erfolgreiche Detektion in IHC: Maus-Dünndarmgewebe, humanes Hirngewebe, humanes Nierengewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16900-1-AP bindet in WB, IHC, ELISA GALNTL2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GALNTL2 fusion protein Ag10434
• Vollständiger Name: UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-like 2
• Berechnetes Molekulargewicht: 639 aa, 73 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC014789
• Gene symbol: GALNTL2
• Gene ID (NCBI): 117248
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GALNTL2, also known as GALNT15, is a member of the polypeptide ppGalNAc-T family. It catalyzes the initial step in O-linked oligosaccharide biosynthesis by transferring an N-acetyl-D-galactosamine residue to serine or threonine residues on protein receptors. Though it shows weaker activity compared to GALNT2, it can transfer up to seven GalNAc residues to the Muc5AC peptide, suggesting cooperative action with other GALNT proteins. GALNTL2 is widely expressed, with high expression in tissues like the small intestine, placenta, spleen, cerebral cortex, and ovary.