GAMT Polyklonaler Antikörper

GAMT Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Rezensionen (1)

Kat-Nr. : 10880-1-AP

Synonyme

GAMT, PIG2, TP53I2

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  WB analysis using 10880-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10880-1-AP (GAMT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 10880-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 10880-1-AP (GAMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10880-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10880-1-AP (GAMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10880-1-AP (GAMT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10880-1-AP (GAMT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10880-1-AP (GAMT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10880-1-AP (GAMT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10880-1-AP (GAMT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10880-1-AP (GAMT antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10880-1-AP (GAMT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10880-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10880-1-AP (GAMT antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Mauslebergewebe, Rattenlebergewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

10880-1-AP bindet in WB, IHC, IF, ELISA GAMT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GAMT fusion protein Ag1327
• Vollständiger Name: guanidinoacetate N-methyltransferase
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC016760
• Gene symbol: GAMT
• Gene ID (NCBI): 2593
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.

Publikationen

Species	Application	Title
mouse	WB	J Agric Food Chem; Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway.; Authors - Sho Nishikawa
mouse	WB	Toxicol Appl Pharmacol; Cytochalasin Q exerts anti-melanoma effect by inhibiting creatine kinase B.; Authors - Yi Lu
rat	WB	Ann Transl Med; Transcriptomics combined with metabolomics analysis of the mechanism of agmatine in the treatment of septic liver injury.; Authors - Ling Huang
mouse	WB,IF	Brain Res; GATM and GAMT synthesize creatine locally throughout the mammalian body and within oligodendrocytes of the brain.; Authors - Steven Andrew Baker
zebrafish		J Fish Biol; Expression patterns of the creatine metabolism-related molecules AGAT, GAMT and CT1 in adult zebrafish Danio rerio.; Authors - Wang L L
mouse	WB	Redox Biol; Unbalanced redox status network as an early pathological event in congenital cataracts; Authors - Eloy Bejarano

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Werner (Verified Customer) (11-28-2017)

13% SDS-PAGE, 10 ug total liver lysate loaded, overnight incubation with primary antibody at 4°C

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver

GAMT Antibody Western Blot, validation (1:2000 dilution) in Mouse liver (Cat no:10880-1-AP)