GAMT Monoklonaler Antikörper

GAMT Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1E7B11

Kat-Nr. : 66322-1-Ig

Synonyme

GAMT, PIG2, TP53I2

---

• 

  WB analysis of fetal human brain using 66322-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 66322-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66322-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66322-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66322-1-Ig (GAMT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, Mauslebergewebe, Hausschwein-Lebergewebe, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below

Produktinformation

66322-1-Ig bindet in WB, ELISA GAMT und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GAMT fusion protein Ag16778
• Vollständiger Name: guanidinoacetate N-methyltransferase
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC016760
• Gene symbol: GAMT
• Gene ID (NCBI): 2593
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.

Publikationen

Species	Application	Title
mouse	WB	Food Funct; The protective effect of polyunsaturated fatty acid intake during pregnancy against embryotoxicity of sodium valproate in mice; Authors - Kelei Li
human	WB	BMC Cardiovasc Disord; PMID: 31888640; Authors - Zhuyin Jia
mouse	WB	Sci Rep; Intratesticular creatine maintains spermatogenesis by defining tight junctions; Authors - Sohei Kuribayashi ; KO Validated
human	WB	Sci Rep; Identification of disulfidptosis-related subtypes in gastric cancer and GAMT is a key gene during disulfidptosis; Authors - Wenxue Wu ; KD Validated