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GAPDH Rekombinanter Antikörper

GAPDH Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H18

Kat-Nr. : 81640-5-PBS

Synonyme

1H18

Filter:
  • Western Blot (WB) analysis of various lysates using GAPDH Recombinant antibody (81640-5-RR)

    WB analysis using 81640-5-RR (same clone as 81640-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81640-5-RR (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81640-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GAPDH Recombinant antibody (81640-5-RR)

    IF Staining of HeLa using 81640-5-RR (same clone as 81640-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GAPDH antibody (81640-5-RR, Clone: 1H18 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 81640-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81640-5-RR

    Affinity and Kinetic Characterization of 81640-5-RR

    Biolayer interferometry (BLl) kinetic assays of 81640-5-RR against Human GAPDH were performed. The affinity constant is 10.4 nM.

Geprüfte Anwendungen

Produktinformation

81640-5-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA GAPDH und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen GAPDH fusion protein Ag0766
Vollständiger Name glyceraldehyde-3-phosphate dehydrogenase
Berechnetes Molekulargewicht 36 kDa
Beobachtetes Molekulargewicht36 kDa
GenBank-ZugangsnummerBC004109
Gene symbol GAPDH
Gene ID (NCBI) 2597
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.