GAPDH Rekombinanter Antikörper

GAPDH Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

CloneNo.

1H18

Rezensionen (2)

Kat-Nr. : 81640-5-RR

Synonyme

1H18

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• 

  WB analysis using 81640-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81640-5-RR (GAPDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 81640-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GAPDH antibody (81640-5-RR, Clone: 1H18 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  Affinity and Kinetic Characterization of 81640-5-RR

  Biolayer interferometry (BLl) kinetic assays of 81640-5-RR against Human GAPDH were performed. The affinity constant is 10.4 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 16 publications below

Produktinformation

81640-5-RR bindet in WB, IF/ICC, ELISA GAPDH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GAPDH fusion protein Ag0766
• Vollständiger Name: glyceraldehyde-3-phosphate dehydrogenase
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC004109
• Gene symbol: GAPDH
• Gene ID (NCBI): 2597
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Publikationen

Species	Application	Title
human,mouse	WB	J Nanobiotechnology; Lemon-derived nanoparticle-functionalized hydrogels regulate macrophage reprogramming to promote diabetic wound healing; Authors - Enyou Jin
human	WB	Int J Biol Sci; FKBP10 Promotes the Muscle Invasion of Bladder Cancer via Lamin A Dysregulation; Authors - Xupeng Zhao
	WB	Respirology; Allele-specific micro-RNA-mediated regulation of ADAM33 in childhood allergic asthma; Authors - Xiang Wen
human	WB	Open Med (Wars); Comprehensive landscape of integrator complex subunits and their association with prognosis and tumor microenvironment in gastric cancer; Authors - Xiaoxia Tong
mouse	WB	J Neuroinflammation; Exercise training upregulates CD55 to suppress complement-mediated synaptic phagocytosis in Parkinson's disease; Authors - Hongkai Yao
human	WB	Ecotoxicol Environ Saf; The role of Drp1 - Pink1 - Parkin - mediated mitophagy in perfluorobutane sulfonate- induced hepatocyte damage; Authors - Yuan Feng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

YINGJIAN (Verified Customer) (07-24-2025)

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: H9C2 cells， liver

FH

YINGJIAN (Verified Customer) (07-24-2025)

This antibody provided clear and well-defined bands at the expected molecular weight in Western blot assays

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: H9C2 cells， liver