GAPDH (Human Specific) Rekombinanter Antikörper

GAPDH (Human Specific) Rekombinant Antikörper für FC (Intra), WB

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2G21

Kat-Nr. : CL647-80570

Synonyme

G3PD, GAPD, GAPDH, OK/SW cl.12

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Galerie der Validierungsdaten

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  WB analysis using CL647-80570

  Various lysates were subjected to SDS PAGE followed by western blot with CL647-80570 (GAPDH (Human Specific) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using CL647-80570

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human GAPDH (Human Specific) (CL647-80570, Clone:2G21) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-80570 bindet in WB, FC (Intra) GAPDH (Human Specific) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GAPDH (Human Specific) fusion protein Ag0766
• Vollständiger Name: glyceraldehyde-3-phosphate dehydrogenase
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC004109
• Gene symbol: GAPDH
• Gene ID (NCBI): 2597
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable. 80570-1-RR is human specific.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C