GAPVD1 Rekombinanter Antikörper

GAPVD1 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241609F12

Kat-Nr. : 84343-3-PBS

Synonyme

241609F12, GAPEX5, GAPex-5, GTPase-activating protein and VPS9 domain-containing protein 1, KIAA1521

---

• 

  WB analysis using 84343-3-RR (same clone as 84343-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84343-3-RR (GAPVD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84343-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 84343-3-RR (same clone as 84343-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84343-3-RR (GAPVD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84343-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 84343-3-RR (same clone as 84343-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84343-3-RR (GAPVD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84343-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84343-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84343-3-RR against Human GAPVD1 were performed. The affinity constant is 1.43 nM.

---

Geprüfte Anwendungen

Produktinformation

84343-3-PBS bindet in WB, IHC, Indirect ELISA GAPVD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GAPVD1 fusion protein Ag10492
• Vollständiger Name: GTPase activating protein and VPS9 domains 1
• Berechnetes Molekulargewicht: 1478 aa, 165 kDa
• Beobachtetes Molekulargewicht: 200-210 kDa
• GenBank-Zugangsnummer: BC021119
• Gene symbol: GAPVD1
• Gene ID (NCBI): 26130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GTPase activating protein and VPS9 domains 1(GAPVD1) is a multifunctional protein involved in cellular processes such as endocytosis, insulin receptor internalization, and GLUT4 trafficking. Its function is critical in maintaining cellular homeostasis and it interacts with various proteins, including those involved in endocytosis and circadian rhythm regulation.