GAPVD1 Rekombinanter Antikörper

GAPVD1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241609F12

Kat-Nr. : 84343-3-RR

Synonyme

241609F12, GAPEX5, GAPex-5, GTPase-activating protein and VPS9 domain-containing protein 1, KIAA1521

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  WB analysis using 84343-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84343-3-RR (GAPVD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 84343-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84343-3-RR (GAPVD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 84343-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84343-3-RR (GAPVD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84343-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84343-3-RR against Human GAPVD1 were performed. The affinity constant is 1.43 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84343-3-RR bindet in WB, IHC, ELISA GAPVD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GAPVD1 fusion protein Ag10492
• Vollständiger Name: GTPase activating protein and VPS9 domains 1
• Berechnetes Molekulargewicht: 1478 aa, 165 kDa
• Beobachtetes Molekulargewicht: 200-210 kDa
• GenBank-Zugangsnummer: BC021119
• Gene symbol: GAPVD1
• Gene ID (NCBI): 26130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GTPase activating protein and VPS9 domains 1(GAPVD1) is a multifunctional protein involved in cellular processes such as endocytosis, insulin receptor internalization, and GLUT4 trafficking. Its function is critical in maintaining cellular homeostasis and it interacts with various proteins, including those involved in endocytosis and circadian rhythm regulation.