GAR1 Polyklonaler Antikörper

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• KD/KO Validated

GAR1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 11711-1-AP

Synonyme

GAR1, NOLA1, snoRNP protein GAR1

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Galerie der Validierungsdaten

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  WB analysis of A375 using 11711-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11711-1-AP (GAR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human skin using 11711-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 11711-1-AP (GAR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 11711-1-AP

  IP Result of anti-GAR1 (IP:11711-1-AP, 3ug; Detection:11711-1-AP 1:300) with A375 cells lysate 6000ug.

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  IHC staining of human skin cancer using 11711-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11711-1-AP (GAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 11711-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11711-1-AP (GAR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, humanes Hautgewebe
• Erfolgreiche IP: A375-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

11711-1-AP bindet in WB, IP, IHC, CoIP, ELISA GAR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GAR1 fusion protein Ag2282
• Vollständiger Name: GAR1 ribonucleoprotein homolog (yeast)
• Berechnetes Molekulargewicht: 217 aa, 22 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC003413
• Gene symbol: GAR1
• Gene ID (NCBI): 54433
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.

Publikationen

Species	Application	Title
human	WB	Mol Cell; The human base excision repair enzyme SMUG1 directly interacts with DKC1 and contributes to RNA quality control.; Authors - Laure Jobert
human	WB	Genome Biol; Human dyskerin binds to cytoplasmic H/ACA-box-containing transcripts affecting nuclear hormone receptor dependence; Authors - Federico Zacchini
human	WB	Front Cell Dev Biol; PARP1 Regulates the Biogenesis and Activity of Telomerase Complex Through Modification of H/ACA-Proteins.; Authors - Nikita V Savelyev
human	WB	Mol Cell Biol; SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization.; Authors - D E MacNeil
human	IHC	Pathology; Expressional analysis of NOLA1, NOLA2, NOLA3 and DKC1, the core proteins in H/ACA riboproteins, in gastric and colorectal cancers.; Authors - Kim Min Sung MS
human	WB	Histochem Cell Biol; Differential requirements for H/ACA ribonucleoprotein components in cell proliferation and response to DNA damage.; Authors - Ping Lin