GAR1 Rekombinanter Antikörper

GAR1 Rekombinant Antikörper für IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240537C11

Kat-Nr. : 83577-1-PBS

Synonyme

240537C11, NOLA1, snoRNP protein GAR1

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  IHC staining of human skin cancer using 83577-1-RR (same clone as 83577-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83577-1-RR (GAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 83577-1-RR (same clone as 83577-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 83577-1-RR (GAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83577-1-RR (same clone as 83577-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GAR1 antibody (83577-1-RR, Clone: 240537C11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

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  FC experiment of K562 using 83577-1-RR (same clone as 83577-1-PBS)

  1x10^6 K562 were intracellularly stained with 0.25 ug GAR1 Recombinant antibody (83577-1-RR, Clone:240537C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83577-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00568-1

  Cytometric bead array standard curve of MP00568-1, GAR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83577-2-PBS. Detection antibody: 83577-1-PBS. Standard: Ag2282. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP00568-2

  Cytometric bead array standard curve of MP00568-2, GAR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83577-3-PBS. Detection antibody: 83577-1-PBS. Standard: Ag2282. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 83577-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83577-1-RR against Human GAR1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83577-1-PBS bindet in IHC, IF/ICC, Cytometric bead array, Indirect ELISA GAR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GAR1 fusion protein Ag2282
• Vollständiger Name: GAR1 ribonucleoprotein homolog (yeast)
• Berechnetes Molekulargewicht: 217 aa, 22 kDa
• GenBank-Zugangsnummer: BC003413
• Gene symbol: GAR1
• Gene ID (NCBI): 54433
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.