GAR1 Rekombinanter Antikörper

GAR1 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240537A2

Kat-Nr. : 83577-4-RR

Synonyme

snoRNP protein GAR1, NOLA1, 240537A2

---

• 

  WB analysis using 83577-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83577-4-RR (GAR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of K562 using 83577-4-RR

  1x10^6 K562 were intracellularly stained with 0.2 ug GAR1 Recombinant antibody (83577-4-RR, Clone:240537A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83577-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83577-4-RR against Human GAR1 were performed. The affinity constant is 1.18 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HeLa-Zellen, Maushautgewebe
• Erfolgreiche Detektion in FC (Intra): K562

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83577-4-RR bindet in WB, FC (Intra), ELISA GAR1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GAR1 fusion protein Ag2282
• Vollständiger Name: GAR1 ribonucleoprotein homolog (yeast)
• Berechnetes Molekulargewicht: 217 aa, 22 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC003413
• Gene symbol: GAR1
• Gene ID (NCBI): 54433
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.