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GAR1 Rekombinanter Antikörper

GAR1 Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240537C11

Kat-Nr. : CL488-83577

Synonyme

snoRNP protein GAR1, NOLA1, 240537C11

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated GAR1 Recombinant ant (CL488-83577)

    IF Staining of HepG2 using CL488-83577

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 GAR1 antibody (CL488-83577, Clone: 240537C11 ) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-83577 bindet in IF/ICC GAR1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen GAR1 fusion protein Ag2282
Vollständiger Name GAR1 ribonucleoprotein homolog (yeast)
Berechnetes Molekulargewicht 217 aa, 22 kDa
GenBank-ZugangsnummerBC003413
Gene symbol GAR1
Gene ID (NCBI) 54433
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL Plus 488 GAR1 antibody CL488-83577Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen