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GARNL1 Polyklonaler Antikörper

GARNL1 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29847-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using GARNL1 Polyclonal antibody (29847-1-AP)

    WB analysis using 29847-1-AP (same clone as 29847-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29847-1-AP (GARNL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29847-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using GARNL1 Polyclonal antibody (29847-1-AP)

    IF Staining of U2OS using 29847-1-AP (same clone as 29847-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GARNL1 antibody (29847-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 29847-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29847-1-PBS bindet in WB, IF/ICC, Indirect ELISA GARNL1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen GARNL1 fusion protein Ag31518
Vollständiger Name GTPase activating Rap/RanGAP domain-like 1
Berechnetes Molekulargewicht230KD
Beobachtetes Molekulargewicht250 kDa
GenBank-ZugangsnummerNM_014990.3
Gene symbol GARNL1
Gene ID (NCBI) 253959
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GARNL1/RalGAPα1, a major α subunit of the Ral-GTPase activating protein in skeletal muscle, as a protein whose phosphorylation and binding to the regulatory 14-3-3 proteins is stimulated by insulin and also by muscle contraction (PMID:24768767).The 100 kDa band maybe an isoform or fragment (BAA74907, 943aa).