GARNL1 Rekombinanter Antikörper

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• KD/KO Validated

GARNL1 Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230165F11

Kat-Nr. : 82971-1-PBS

Synonyme

Tuberin like protein 1, RALGAPA1, p240, KIAA0884, GRIPE

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  WB analysis using 82971-1-RR (same clone as 82971-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82971-1-RR (GARNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 82971-1-RR (same clone as 82971-1-PBS)

  WB result of GARNL1 antibody (82971-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GARNL1 transfected HEK-293 cells. This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

• 

  FC experiment of U2OS using 82971-1-RR (same clone as 82971-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug GARNL1 Recombinant antibody (82971-1-RR, Clone:230165F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82971-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82971-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82971-1-RR against Human GARNL1 were performed. The affinity constant is 99.6 pM.

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Geprüfte Anwendungen

Produktinformation

82971-1-PBS bindet in WB, FC (Intra), Indirect ELISA GARNL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GARNL1 fusion protein Ag31518
• Vollständiger Name: GTPase activating Rap/RanGAP domain-like 1
• Berechnetes Molekulargewicht: 230KD
• Beobachtetes Molekulargewicht: 260 kDa
• GenBank-Zugangsnummer: NM_014990.3
• Gene symbol: GARNL1
• Gene ID (NCBI): 253959
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GARNL1/RalGAPα1, a major α subunit of the Ral-GTPase activating protein in skeletal muscle, is a protein whose phosphorylation and binding to the regulatory 14-3-3 proteins is stimulated by insulin and also by muscle contraction (PMID:24768767).