GARS Monoklonaler Antikörper

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• KD/KO Validated

GARS Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H9D3

Kat-Nr. : 67893-1-PBS

Synonyme

AP 4 A synthetase, CMT2D, DSMAV, GARS, Glycine tRNA ligase, glycyl tRNA synthetase, GlyRS, HMN5, SMAD1

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  WB analysis using 67893-1-Ig (same clone as 67893-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67893-1-Ig (GARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67893-1-Ig (same clone as 67893-1-PBS)

  WB result of GARS antibody (67893-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GARS transfected HepG2 cells. This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 67893-1-Ig (same clone as 67893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67893-1-Ig (GARS antibody) at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 67893-1-Ig (same clone as 67893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 67893-1-Ig (GARS antibody) at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67893-1-Ig (same clone as 67893-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GARS antibody (67893-1-Ig, Clone: 2H9D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67893-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67893-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GARS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GARS fusion protein Ag8432
• Vollständiger Name: glycyl-tRNA synthetase
• Berechnetes Molekulargewicht: 739 aa, 83 kDa
• Beobachtetes Molekulargewicht: 75-80 kDa
• GenBank-Zugangsnummer: BC007755
• Gene symbol: GARS
• Gene ID (NCBI): 2617
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycyl-tRNA synthetase 1 (GARS, GeneID:2617) is one of the aminoacyl-tRNA synthetases. It is essential for protein synthesis in all cells and tissues (PMID: 17035524) and charges tRNA molecules with glycine by catalyzing the ATP-dependent ligation of glycine to the 3'-end of it's cognate tRNA via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PMID: 17544401, PMID: 28675565, PMID: 24898252) . GARS is located in cytoplasm, but also can be secreted from cells and as an extracellular protein which may influence cell signaling. Mutation of GARS causes peripheral neuropathies such as CMT2D, SMA (PMID: 26503042, PMID: 32181591) , or associates with cancer. Two transcript variants encoding different isoforms have been found for this gene. The molecular weight of isoform2 is about 78 kDa.