GART Polyklonaler Antikörper

GART Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (1)

Kat-Nr. : 13659-1-AP

Synonyme

AIR synthase, AIRS, GAR transformylase, GARS, GART, GARTF, PAIS, PGFT, PRGS

---

Galerie der Validierungsdaten

• 

  WB analysis using 13659-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13659-1-AP (GART antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13659-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13659-1-AP (GART antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13659-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13659-1-AP (GART antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13659-1-AP

  IP Result of anti-GART (IP:13659-1-AP, 4ug; Detection:13659-1-AP 1:500) with HepG2 cells lysate 1600ug.

• 

  IHC staining of human cervical cancer using 13659-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13659-1-AP (GART antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13659-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13659-1-AP (GART antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13659-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13659-1-AP (GART antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13659-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA GART und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GART fusion protein Ag4555
• Vollständiger Name: phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
• Berechnetes Molekulargewicht: 1010 aa, 108 kDa
• Beobachtetes Molekulargewicht: 108 kDa, 46 kDa
• GenBank-Zugangsnummer: BC038958
• Gene symbol: GART
• Gene ID (NCBI): 2618
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GART is a trifunctional polypeptide which has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity that is required for de novo purine biosynthesis. GART has 2 isoforms with molecular mass of 108 kDa and 46 kDa due to alternative splicing.

Publikationen

Species	Application	Title
		Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
mouse	WB	Nat Commun; Purine nucleotide depletion prompts cell migration by stimulating the serine synthesis pathway.; Authors - Mona Hoseini Soflaee
human	WB	EMBO J; UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism.; Authors - Xing Feng
human	WB	Cell Rep; Purine Nucleotide Availability Regulates mTORC1 Activity through the Rheb GTPase.; Authors - Natasha Emmanuel
human	WB	Nat Metab; Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.; Authors - Diem H Tran

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Hua (Verified Customer) (08-26-2019)

Works well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HepG2, mouse liver, mouse lung, mouse hippocampus

GART Antibody Western Blot, validation (1:500 dilution) in HepG2, mouse liver, mouse lung, mouse hippocampus (Cat no:13659-1-AP)