GAS1 Monoklonaler Antikörper

GAS1 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2H8E1

Kat-Nr. : 67181-1-Ig

Synonyme

GAS 1, GAS1, growth arrest 1

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  WB analysis using 67181-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67181-1-Ig (GAS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 67181-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67181-1-Ig (GAS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 67181-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67181-1-Ig (GAS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 67181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67181-1-Ig (GAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 67181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67181-1-Ig (GAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Hausschwein-Hirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67181-1-Ig bindet in WB, IHC, ELISA GAS1 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GAS1 fusion protein Ag12259
• Vollständiger Name: growth arrest-specific 1
• Berechnetes Molekulargewicht: 345 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC074908
• Gene symbol: GAS1
• Gene ID (NCBI): 2619
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAS1 is involved in growth suppression. It is an integral plasma membrane protein whose expression is linked to growth arrest. It can be used as a novel therapeutic candidate for gastric cancer.