GAS2L1 Monoklonaler Antikörper

GAS2L1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C6D5

Kat-Nr. : 68051-1-PBS

Synonyme

GAR22, GAS2 like protein 1, GAS2L1, growth arrest 2 like 1

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  WB analysis using 68051-1-Ig (same clone as 68051-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68051-1-Ig (GAS2L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 68051-1-Ig (same clone as 68051-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 68051-1-Ig (GAS2L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68051-1-Ig (same clone as 68051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68051-1-Ig (GAS2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 68051-1-Ig (same clone as 68051-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68051-1-Ig (GAS2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68051-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68051-1-PBS bindet in WB, IHC, Indirect ELISA GAS2L1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GAS2L1 fusion protein Ag31232
• Vollständiger Name: growth arrest-specific 2 like 1
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC011047
• Gene symbol: GAS2L1
• Gene ID (NCBI): 10634
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GAS2L1(growth arrest‐specific 2‐like 1) is a component of the cytoskeleton that mediates the interaction between microtubules and microfilament (PMID: 34555082). GAS2L1 contains an N‐terminal actin‐binding calponin homology (CH) domain, a MT lattice‐binding Gas2‐related (GAR) domain and a C‐terminal SxIP motif, which mediates the interaction with MT plus ends via EB proteins. GAS2L1 regulates microtubule dynamics and stability by interacting with microtubule plus-end tracking proteins, such as MAPRE1, to regulate microtubule growth along actin stress fibers (PubMed:24706950).