GAS6 Polyklonaler Antikörper

GAS6 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 13795-1-AP

Synonyme

AXLLG, AXSF, GAS 6, GAS6, growth arrest 6

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Galerie der Validierungsdaten

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  WB analysis of human urine using 13795-1-AP

  human urine sample were subjected to SDS PAGE followed by western blot with 13795-1-AP (GAS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 13795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13795-1-AP (GAS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 13795-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 13795-1-AP (GAS6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 13795-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13795-1-AP (GAS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13795-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13795-1-AP (GAS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 13795-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13795-1-AP (GAS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 13795-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 13795-1-AP (GAS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13795-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13795-1-AP (GAS6 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humane Urinprobe, DU 145-Zellen, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

13795-1-AP bindet in WB, IHC, IF, ELISA GAS6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GAS6 fusion protein Ag4617
• Vollständiger Name: growth arrest-specific 6
• Berechnetes Molekulargewicht: 678 aa, 75 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC038984
• Gene symbol: GAS6
• Gene ID (NCBI): 2621
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Growth arrest-specific protein 6 (GAS6) is also named as AXLLG. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses (PMID:12364394, PMID:18840707). It can bridge virus envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entry by apoptotic mimicry (PMID:21501828).

Publikationen

Species	Application	Title
human	WB	Int J Cancer; Therapeutic Activity of DCC-2036, a Novel Tyrosine Kinase Inhibitor, against Triple-Negative Breast Cancer Patient-Derived Xenografts by Targeting AXL/MET.; Authors - Yingying Shen
human	WB	Front Cell Infect Microbiol; Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells.; Authors - Fengxue Geng
human	WB	FASEB J; Anti-human serum albumin autoantibody may be involved in the pathogenesis of autoimmune bullous skin diseases.; Authors - Hua Qian
human	WB,IHC	Cancer Gene Ther; Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.; Authors - Minhai Dong
mouse	WB	Transplantation; Activated AXL Protects Against Hepatic Ischemia-reperfusion Injury by Upregulating SOCS-1 Expression.; Authors - Zhen Wang
human	WB	Mol Immunol; The role of the Gas6/TAM signal pathway in the LPS-induced pulmonary epithelial cells injury; Authors - Yujing Cheng