GATA1 Polyklonaler Antikörper

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• KD/KO Validated

GATA1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10917-2-AP

Synonyme

ERYF1, Erythroid transcription factor, GATA 1, GATA binding factor 1, GATA1, GF 1, GF1, NF E1 DNA binding protein, NFE1, XLTT

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Galerie der Validierungsdaten

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  WB analysis using 10917-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10917-2-AP (GATA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10917-2-AP

  WB result of GATA1 antibody (10917-2-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GATA1 transfected K-562 cells.

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  WB analysis of Raji using 10917-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10917-2-AP (GATA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10917-2-AP

  IP Result of anti-GATA1 (IP:10917-2-AP, 4ug; Detection:10917-2-AP 1:500) with K-562 cells lysate 4000ug.

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  IHC staining of mouse testis using 10917-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10917-2-AP (GATA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10917-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10917-2-AP (GATA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, K-562-Zellen, NIH/3T3-Zellen, Raji-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 4 publications below

Produktinformation

10917-2-AP bindet in WB, IP, IHC, ELISA GATA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GATA1 fusion protein Ag1350
• Vollständiger Name: GATA binding protein 1 (globin transcription factor 1)
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa, 40-45 kDa
• GenBank-Zugangsnummer: BC009797
• Gene symbol: GATA1
• Gene ID (NCBI): 2623
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757]. GATA1 exists two isoform with the molecular weight 50-55 kDa and 40-45 kDa (PMID: 31189107 ).

Publikationen

Species	Application	Title
human	WB,IHC	Oncogene; microRNA-23a, -27a and -24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia.; Authors - R Su
human	WB	Front Immunol; FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome.; Authors - Chunlin Wang
mouse	IHC	Genes (Basel); Proof of Gene Doping in a Mouse Model with a Human Erythropoietin Gene Transferred Using an Adenoviral Vector.; Authors - Takehito Sugasawa
human	WB,IHC	Oncotarget; The transcription factor GATA1 and the histone methyltransferase SET7 interact to promote VEGF-mediated angiogenesis and tumor growth and predict clinical outcome of breast cancer.; Authors - Yanan Zhang
human	WB	Biomed Pharmacother; Xanthotoxin, a novel inducer of platelet formation, promotes thrombocytopoiesis via IL-1R1 and MEK/ERK signaling; Authors - Jia Lai
human	WB,IHC	Mol Cell Biochem; GATA1 promotes colorectal cancer cell proliferation, migration and invasion via activating AKT signaling pathway.; Authors - Junhui Yu ; KD Validated