GATA1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

GATA1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

CloneNo.

5E2A8

Kat-Nr. : 60011-1-Ig

Synonyme

5E2A8, ERYF1, GATA-1, GATA-binding factor 1, GF1

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  WB analysis using 60011-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 60011-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 60011-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 60011-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 60011-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 60011-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 60011-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GATA1 antibody (60011-1-Ig, Clone: 5E2A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Jurkat-Zellen, K-562-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

60011-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA GATA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GATA1 fusion protein Ag1350
• Vollständiger Name: GATA binding protein 1 (globin transcription factor 1)
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC009797
• Gene symbol: GATA1
• Gene ID (NCBI): 2623
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].

Publikationen

Species	Application	Title
human	IP	Blood; PML4 facilitates erythroid differentiation by enhancing the transcriptional activity of GATA-1.; Authors - Wu Jie J
human	IF	Development; The PAX-SIX-EYA-DACH network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis.; Authors - T Michael Creed ; KO Validated
human	WB	Biochem J; ZNF16 (HZF1) promotes erythropoiesis and megakaryocytopoiesis via its regulation on c-KIT gene.; Authors - Chen Jing J
human	WB	J Cell Mol Med; 1,25(OH)2 D3 blocks IFNβ production through regulating STING in epithelial layer of oral lichen planus.; Authors - Xuejun Ge
human	WB	Cell Death Discov; JAC1 suppresses proliferation of breast cancer through the JWA/p38/SMURF1/HER2 signaling.; Authors - Yanlin Ren
human	WB	Cell Death Dis; GATA3 recruits UTX for gene transcriptional activation to suppress metastasis of breast cancer.; Authors - Wenqian Yu