GATA2 Monoklonaler Antikörper

GATA2 Monoklonal Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B5A1

Kat-Nr. : 67722-1-PBS

Synonyme

2B5A1, Endothelial transcription factor GATA-2, GATA binding protein 2, GATA-binding protein 2, NFE1B

---

• 

  WB analysis using 67722-1-Ig (same clone as 67722-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of bEnd.3 using 67722-1-Ig (same clone as 67722-1-PBS)

  bEnd.3 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67722-1-Ig (same clone as 67722-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 67722-1-Ig (same clone as 67722-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67722-1-Ig (same clone as 67722-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67722-1-Ig (same clone as 67722-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67722-1-Ig (same clone as 67722-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67722-1-Ig (same clone as 67722-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 67722-1-Ig (same clone as 67722-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HUVEC using 67722-1-Ig (same clone as 67722-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 67722-1-Ig (same clone as 67722-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67722-1-Ig (same clone as 67722-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67722-1-Ig (GATA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 67722-1-Ig (same clone as 67722-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using GATA2 antibody (67722-1-Ig, Clone: 2B5A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 67722-1-Ig (same clone as 67722-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human GATA2 (67722-1-Ig, Clone:2B5A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67722-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67722-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA GATA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GATA2 fusion protein Ag1557
• Vollständiger Name: GATA binding protein 2
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC018988
• Gene symbol: GATA2
• Gene ID (NCBI): 2624
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GATA2 is a member of GATA family of transcription factors that contains zinc fingers in DNA binding domain. GATA2 acts as a transcriptinal activator that regulates endothelin-1 expression in endothelial cells. Together with GATA3, GATA2 regulates adipocyte differentiation through molecular control of the preadpocyte-adipocyte transition. It also affect the activity of Rho inhibitor p190RhoGAP that controls capillary network formation in vitro and retinal angiogenesis in vivo.