GATA3 Polyklonaler Antikörper

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• KD/KO Validated

GATA3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

29

Kat-Nr. : 10417-1-AP

Synonyme

GATA binding factor 3, GATA binding protein 3, GATA3, HDR

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 10417-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10417-1-AP (GATA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10417-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10417-1-AP (GATA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10417-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10417-1-AP (GATA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 10417-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10417-1-AP (GATA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10417-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10417-1-AP (GATA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 19 publications below
• ChIP: See 1 publications below

Produktinformation

10417-1-AP bindet in WB, IHC, ChIP, ELISA GATA3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GATA3 fusion protein Ag0174
• Vollständiger Name: GATA binding protein 3
• Berechnetes Molekulargewicht: 443 aa, 48 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC003070
• Gene symbol: GATA3
• Gene ID (NCBI): 2625
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression. This is a rabbit polyclonal antibody raised against part of C-terminus of human GATA3.

Publikationen

Species	Application	Title
human		Cancer Cell; Lunatic fringe deficiency cooperates with the Met/Caveolin gene amplicon to induce basal-like breast cancer.; Authors - Keli Xu
		Cancer Res; Cooperation between Pik3ca and p53 mutations in mouse mammary tumor formation.; Authors - Adams Jessica R JR
	WB	Oxid Med Cell Longev; Androgen Plays a Potential Novel Hormonal Therapeutic Role in Th17 Cells Predominant Neutrophilic Severe Asthma by Attenuating BECs Regulated Th17 Cells Differentiation via MBD2 Expression; Authors - Binaya Wasti
human	WB	Cell Death Dis; Calreticulin promotes EGF-induced EMT in pancreatic cancer cells via Integrin/EGFR-ERK/MAPK signaling pathway.; Authors - Weiwei Sheng
human		Development; The inhibitor of histone acetyltransferases Nir regulates epidermis development.; Authors - Delphine Duteil
human	WB	Int J Cardiol; Endothelial microparticles exert differential effects on functions of Th1 in patients with acute coronary syndrome.; Authors - Lu Yongguang Y

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