GATA6 Rekombinanter Antikörper

GATA6 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242325B6

Kat-Nr. : 84987-3-RR

Synonyme

242325B6, GATA-binding factor 6, Transcription factor GATA 6, Transcription factor GATA-6

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  WB analysis using 84987-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84987-3-RR (GATA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84987-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GATA6 antibody (84987-3-RR, Clone: 242325B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HEK-293 using 84987-3-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GATA6 Recombinant antibody (84987-3-RR, Clone:242325B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, SGC-7901-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84987-3-RR bindet in WB, IF/ICC, FC (Intra), ELISA GATA6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GATA binding protein 6
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60-64 kDa
• GenBank-Zugangsnummer: NM_005257
• Gene symbol: GATA6
• Gene ID (NCBI): 2627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GATA6 is a member of the GATA family of zinc-finger transcriptional regulators, which play crucial roles in the regulation of cell growth, differentiation, survival, and maintenance of body functions. Named after the conserved base sequence (G/A)GATA(A/T), GATA6 features conserved tandem zinc fingers and is essential in coordinating the development and precise gene regulation of diverse tissues, including the heart and gastrointestinal tract. GATA6 recognizes the sequence (A/T/C)GATA(A/T)A and interacts with other transcriptional regulators through its zinc-finger domain.