GBE1 Rekombinanter Antikörper

GBE1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242705G3

Kat-Nr. : 85143-5-RR

Synonyme

1,4-alpha-glucan-branching enzyme, 242705G3, APBD, EC:2.4.1.18, GBE

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  WB analysis using 85143-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85143-5-RR (GBE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85143-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85143-5-RR (GBE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85143-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85143-5-RR against Human GBE1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, Mauslebergewebe, Mauslungengewebe, Rattenlebergewebe, Rattenlungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85143-5-RR bindet in WB, ELISA GBE1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GBE1 fusion protein Ag14154
• Vollständiger Name: glucan (1,4-alpha-), branching enzyme 1
• Berechnetes Molekulargewicht: 702 aa, 80 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC012098
• Gene symbol: GBE1
• Gene ID (NCBI): 2632
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GBE1 (glucan (1,4-alpha-), branching enzyme 1) is essential for the globular and branched structure of glycogen, increasing solubility by creating a hydrophilic surface and reducing intracellular osmotic pressure. It may serve as a potential therapeutic target for treating LUAD(PMID: 31221150). The full sequence can be further processed into a mature form of 75 kDa.