GBP1 Rekombinanter Antikörper

GBP1 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240634G8

Kat-Nr. : 83751-6-RR

Synonyme

Guanine nucleotide-binding protein 1, GTP-binding protein 1, GBP-1, GBP 1, EC:3.6.1.-

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  WB analysis using 83751-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83751-6-RR (GBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83751-6-RR

  1x10^6 HeLa cells untreated (dashed lines) or treated with IFN gamma (red) were intracellularly stained with 0.25 ug GBP1 Recombinant antibody (83751-6-RR, Clone: 240634G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83751-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83751-6-RR against Human GBP1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, HUVEC-Zellen
• Erfolgreiche Detektion in FC (Intra): IFN gamma (100ng/ml 24h) HeLa cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83751-6-RR bindet in WB, FC (Intra), ELISA GBP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GBP1 fusion protein Ag7562
• Vollständiger Name: GTP binding protein 1
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC002666
• Gene symbol: GBP1
• Gene ID (NCBI): 2633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Guanylate binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. The expression of GBP1 is induced by interferon and GBP1 is characterized by its ability to specifically bind guanine nucleotides such as GMP, GDP, and GTP and its ability to hydrolyze GTP to GDP and GMP. GBP1 is induced in response to type I and type II interferons and as such plays a role in protective immunity against a spectrum of intracellular pathogens ranging from viruses to bacteria to protozoa, such as negative-strand RNA Rhabdovirus, vesicular stomatitis virus, and the positive-strand RNA Picornovirus, encephalomyocarditis virus in cultured cells, the inhibition of Chlamydia trachomatis, Toxoplasma gondii, and Salmonella enterica.