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GBX2 Polyklonaler Antikörper

GBX2 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21639-1-PBS

Synonyme

Homeobox protein GBX-2, Gastrulation and brain-specific homeobox protein 2

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  • Western Blot (WB) analysis of HeLa cells using GBX2 Polyclonal antibody (21639-1-AP)

    WB analysis of HeLa using 21639-1-AP (same clone as 21639-1-PBS)

    WB result of GBX2 antibody (21639-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GBX2 transfected HeLa cells. This data was developed using the same antibody clone with 21639-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse stomach tissue using GBX2 Polyclonal antibody (21639-1-AP)

    WB analysis of mouse stomach using 21639-1-AP (same clone as 21639-1-PBS)

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21639-1-AP (GBX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21639-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using GBX2 Polyclonal antibody (21639-1-AP)

    IP experiment of HeLa using 21639-1-AP (same clone as 21639-1-PBS)

    IP result of anti-GBX2 (IP:21639-1-AP, 4ug; Detection:21639-1-AP 1:300) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 21639-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GBX2 Polyclonal antibody (21639-1-AP)

    IF Staining of HeLa using 21639-1-AP (same clone as 21639-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GBX2 antibody (21639-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21639-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

21639-1-PBS bindet in WB, IF/ICC, IP, ELISA GBX2 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen GBX2 fusion protein Ag16288
Vollständiger Name gastrulation brain homeobox 2
Berechnetes Molekulargewicht 348 aa, 37 kDa
Beobachtetes Molekulargewicht 33-37 kDa
GenBank-ZugangsnummerBC137448
Gene symbol GBX2
Gene ID (NCBI) 2637
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Homeobox protein GBX-2 (GBX-2) is a homeobox gene essential for normal development of the midbrain and the anterior hindbrain. It mainly expressed in the brain, and located in nucleoplasm. It is presumably a transcription factor that is likely to regulate the expression of genes involved in establishing early A/P pattern in the neural plate (PMID: 9247335).