GCAT Polyklonaler Antikörper

GCAT Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 16317-1-AP

Synonyme

2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial, AKB ligase, Aminoacetone synthase, EC:2.3.1.29, Glycine acetyltransferase

---

• 

  WB analysis using 16317-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16317-1-AP (GCAT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 16317-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16317-1-AP (GCAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 16317-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 16317-1-AP (GCAT antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 16317-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16317-1-AP (GCAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 16317-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16317-1-AP (GCAT antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:10000-1:40000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

16317-1-AP bindet in WB, IHC, ELISA GCAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GCAT fusion protein Ag9367
• Vollständiger Name: glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase)
• Berechnetes Molekulargewicht: 419 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC014457
• Gene symbol: GCAT
• Gene ID (NCBI): 23464
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GCAT (Glycine acetyltransferase) is an enzyme involved in the metabolism of amino acids, specifically in the degradation of L-threonine to glycine. GCAT expression can be regulated epigenetically, and its downregulation has been linked to mitochondrial respiration defects, particularly in aging cells (PMID: 26000717).

Publikationen

Species	Application	Title
mouse	WB	Life Sci Alliance; Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.; Authors - Shengya Tian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Lana (Verified Customer) (10-04-2019)

SDS-PAGE: 15 ug/ul RIPA lysate of the mitochondrial fraction of ST-Hdh-Q7/Q7 cells. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4CSecondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: ST-Hdh mouse striatal cell line

GCAT Antibody Western Blot, validation (1:2000 dilution) in ST-Hdh mouse striatal cell line (Cat no:16317-1-AP)