GCLC Polyklonaler Antikörper

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• KD/KO Validated

GCLC Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12601-1-PBS

Synonyme

EC:6.3.2.2, Gamma-ECS, Gamma-glutamylcysteine synthetase, GLCL, GLCLC

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  WB analysis using 12601-1-AP (same clone as 12601-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 12601-1-AP (same clone as 12601-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 12601-1-AP (same clone as 12601-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 12601-1-AP (same clone as 12601-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP (GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 12601-1-AP (same clone as 12601-1-PBS)

  IP result of anti-GCLC (IP:12601-1-AP, 4ug; Detection:12601-1-AP 1:5000) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12601-1-AP (same clone as 12601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12601-1-AP (GCLC Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12601-1-AP (same clone as 12601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12601-1-AP (GCLC Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 12601-1-AP (same clone as 12601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12601-1-AP (GCLC antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 12601-1-AP (same clone as 12601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12601-1-AP (GCLC antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12601-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12601-1-PBS bindet in WB, IHC, IP, ELISA GCLC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GCLC fusion protein Ag3252
• Vollständiger Name: glutamate-cysteine ligase, catalytic subunit
• Berechnetes Molekulargewicht: 637 aa, 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC022487
• Gene symbol: GCLC
• Gene ID (NCBI): 2729
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family.It is also named as GLCL, GLCLC.It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis.GCLC) gene may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).