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GCNA Rekombinanter Antikörper

GCNA Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241428A9

Kat-Nr. : 84124-4-PBS

Synonyme

ACRC, Germ cell nuclear antigen, Germ cell nuclear acidic protein, Germ cell nuclear acidic peptidase, Acidic repeat-containing protein

Filter:
  • Western Blot (WB) analysis of various lysates using GCNA Recombinant antibody (84124-4-RR)

    WB analysis using 84124-4-RR (same clone as 84124-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84124-4-RR (GCNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84124-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using GCNA Recombinant antibody (84124-4-RR)

    IF Staining of LNCaP using 84124-4-RR (same clone as 84124-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using GCNA antibody (84124-4-RR, Clone: 241428A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84124-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84124-4-RR

    Affinity and Kinetic Characterization of 84124-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84124-4-RR against Human GCNA were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

84124-4-PBS bindet in WB, IF/ICC, Indirect ELISA GCNA und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen GCNA fusion protein Ag21895
Vollständiger Name acidic repeat containing
Berechnetes Molekulargewicht 691 aa, 76 kDa
Beobachtetes Molekulargewicht90 kDa
GenBank-ZugangsnummerBC136259
Gene symbol GCNA
Gene ID (NCBI) 93953
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GCNA, also known as ACRC, belongs to the serine-aspartate repeat-containing protein (SDr) family. GCNA has remained tightly associated with sexual reproduction showing enriched expression within germ cells in both invertebrate and vertebrate species. GCNA has recently been implicated in resolving DPCs in germ cells and early embryos. GCNA is targeted to SUMOylated DPC proteins through its SUMO-interacting motifs (SIMs), reinforcing the importance of SUMOylation in DPC repair. The amino acid sequence homology of GCNA in humans and mice is very low. The calculated molecular weight of GCNA is 76 kDa. Sometimes higher molecular weight around 90 kDa can also be observed, which may be caused by SUMO modification (PMID: 31839537, 32329115).