GCNA Rekombinanter Antikörper

GCNA Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241428A9

Kat-Nr. : 84124-4-RR

Synonyme

ACRC, Germ cell nuclear antigen, Germ cell nuclear acidic protein, Germ cell nuclear acidic peptidase, Acidic repeat-containing protein

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  WB analysis using 84124-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84124-4-RR (GCNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of LNCaP using 84124-4-RR

  Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using GCNA antibody (84124-4-RR, Clone: 241428A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84124-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84124-4-RR against Human GCNA were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, A549-Zellen, DU 145-Zellen, HepG2-Zellen, humanes Hodengewebe, MCF-7-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84124-4-RR bindet in WB, IF/ICC, ELISA GCNA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GCNA fusion protein Ag21895
• Vollständiger Name: acidic repeat containing
• Berechnetes Molekulargewicht: 691 aa, 76 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC136259
• Gene symbol: GCNA
• Gene ID (NCBI): 93953
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GCNA, also known as ACRC, belongs to the serine-aspartate repeat-containing protein (SDr) family. GCNA has remained tightly associated with sexual reproduction showing enriched expression within germ cells in both invertebrate and vertebrate species. GCNA has recently been implicated in resolving DPCs in germ cells and early embryos. GCNA is targeted to SUMOylated DPC proteins through its SUMO-interacting motifs (SIMs), reinforcing the importance of SUMOylation in DPC repair. The amino acid sequence homology of GCNA in humans and mice is very low. The calculated molecular weight of GCNA is 76 kDa. Sometimes higher molecular weight around 90 kDa can also be observed, which may be caused by SUMO modification (PMID: 31839537, 32329115).