GCNF Polyklonaler Antikörper

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GCNF Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12712-1-PBS

Synonyme

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  WB analysis of PC-3 using 12712-1-AP (same clone as 12712-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12712-1-AP (GCNF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12712-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 12712-1-AP (same clone as 12712-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12712-1-AP (GCNF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12712-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 12712-1-AP (same clone as 12712-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12712-1-AP (GCNF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12712-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 12712-1-AP (same clone as 12712-1-PBS)

  IP result of anti-GCNF (IP:12712-1-AP, 4ug; Detection:12712-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 12712-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12712-1-AP (same clone as 12712-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12712-1-AP (GCNF antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12712-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12712-1-AP (same clone as 12712-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12712-1-AP (GCNF antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12712-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12712-1-PBS bindet in WB, IHC, IP, Indirect ELISA GCNF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GCNF fusion protein Ag3395
• Vollständiger Name: nuclear receptor subfamily 6, group A, member 1
• Berechnetes Molekulargewicht: 475 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC030600
• Gene symbol: GCNF
• Gene ID (NCBI): 2649
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR6A1 (Nuclear Receptor Subfamily 6 Group A member), also known as GCNF (Germ Cell Nuclear Factor), is an orphan member of the nuclear receptor gene superfamily. It has been shown to be expressed in the nervous system during development and during specific stages in maturing germ cells of the ovary and testis in the adult, and has probable roles in gametogenesis, neurogenesis, and normal embryonic development during gastrulation. Inactivation of GCNF in mouse results in abnormal posterior development, impaired midbrain development, insufficient closure of the neural tube, and eventual embryonic death. GCNF has been shown to be a repressor of OCT-4 and plays a critical role in the control of gene expression during embryogenesis and spermatogenesis. This antibody specifically reacts with the 55kd NR6A1 protein.