GCSH Polyklonaler Antikörper

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• KD/KO Validated

GCSH Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 16726-1-AP

Synonyme

NKH, MMDS7, Glycine cleavage system H protein, mitochondrial, GCE

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  WB analysis of HEK-293 using 16726-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16726-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16726-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16726-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16726-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 16726-1-AP

  IP result of anti-GCSH (IP:16726-1-AP, 3ug; Detection:16726-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human ovary cancer using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16726-1-AP (GCSH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16726-1-AP (GCSH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16726-1-AP (GCSH Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16726-1-AP (GCSH Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of rat ovary using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary slide using 16726-1-AP (GCSH Antibody) at dilution of 1:50.

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  IHC staining of rat ovary using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary slide using 16726-1-AP (GCSH Antibody) at dilution of 1:50.

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  IHC staining of human ovary using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16726-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 16726-1-AP

  Immunofluorescent analysis of MCF-7 cells, using GCSH antibody 16726-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 16726-1-AP

  Immunofluorescent analysis of HepG2 cells, using GCSH antibody 16726-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Lebergewebe, Maushirngewebe, Mausnierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: human ovary cancer tissue, humanes Nierengewebe, humanes Lebergewebe, humanes Eierstockgewebe, humanes Hautgewebe, Ratten-Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

16726-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA GCSH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GCSH fusion protein Ag10174
• Vollständiger Name: glycine cleavage system protein H (aminomethyl carrier)
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC000790
• Gene symbol: GCSH
• Gene ID (NCBI): 2653
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encodes a precursor protein of 173 amino acids and a mature protein of 125 amino acids. The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein(PMID:2211640). Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH).

Publikationen

Species	Application	Title
		Science; Copper induces cell death by targeting lipoylated TCA cycle proteins.; Authors - Peter Tsvetkov
human	WB	Nat Commun; Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.; Authors - Jiliang Xia ; KD Validated
human	WB	J Biol Chem; Acute loss of iron-sulfur clusters results in metabolic reprogramming and generation of lipid droplets in mammalian cells.; Authors - Daniel R Crooks
human	WB,IHC,IF	Sci Rep; GCSH antisense regulation determines breast cancer cells' viability.; Authors - Anna Adamus
human	WB	J Biol Chem; FDX1 regulates cellular protein lipoylation through direct binding to LIAS; Authors - Margaret B Dreishpoon
human	WB	PLoS One; Amino Acid starvation has opposite effects on mitochondrial and cytosolic protein synthesis.; Authors - Mark A Johnson