GCSH Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GCSH Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16726-1-PBS

Synonyme

NKH, MMDS7, Glycine cleavage system H protein, mitochondrial, GCE

---

• 

  WB analysis of HEK-293 using 16726-1-AP (same clone as 16726-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 16726-1-AP (same clone as 16726-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 16726-1-AP (same clone as 16726-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16726-1-AP (same clone as 16726-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 16726-1-AP (same clone as 16726-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 16726-1-AP (GCSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse kidney using 16726-1-AP (same clone as 16726-1-PBS)

  IP result of anti-GCSH (IP:16726-1-AP, 3ug; Detection:16726-1-AP 1:1000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16726-1-AP (GCSH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16726-1-AP (GCSH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16726-1-AP (GCSH Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16726-1-AP (GCSH Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat ovary using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary slide using 16726-1-AP (GCSH Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat ovary using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary slide using 16726-1-AP (GCSH Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16726-1-AP (same clone as 16726-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16726-1-AP (GCSH antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 16726-1-AP (same clone as 16726-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using GCSH antibody 16726-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16726-1-AP (same clone as 16726-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using GCSH antibody 16726-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16726-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

16726-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA GCSH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GCSH fusion protein Ag10174
• Vollständiger Name: glycine cleavage system protein H (aminomethyl carrier)
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC000790
• Gene symbol: GCSH
• Gene ID (NCBI): 2653
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encodes a precursor protein of 173 amino acids and a mature protein of 125 amino acids. The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein(PMID:2211640). Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH).