GDF-15 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GDF-15 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(36)

Rezensionen (1)

Kat-Nr. : 27455-1-AP

Synonyme

GDF15, GDF 15, growth differentiation factor 15, Growth/differentiation factor 15, MIC-1

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• 

  WB analysis using 27455-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27455-1-AP (GDF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27455-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27455-1-AP (GDF-15 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27455-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27455-1-AP (GDF-15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27455-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27455-1-AP (GDF-15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27455-1-AP

  WB result of GDF15 antibody (27455-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GDF15 transfected HepG2 cells.

• 

  IHC staining of human placenta using 27455-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27455-1-AP (GDF-15 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 27455-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27455-1-AP (GDF-15 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, LNCaP-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 16 publications below
• WB: See 42 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Produktinformation

27455-1-AP bindet in WB, IHC, IF, ELISA GDF-15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GDF-15 fusion protein Ag26760
• Vollständiger Name: growth differentiation factor 15
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC008962
• Gene symbol: GDF15
• Gene ID (NCBI): 9518
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Growth differentiation factor 15 (GDF15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily that regulates inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Dual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer.; Authors - Tiziano Bernasocchi
human	WB	Cancers (Basel); GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.; Authors - Chiara Bellio ; KD Validated
human	WB	Commun Biol; Melatonin affects trophoblast epithelial-to-mesenchymal transition and oxidative damage resistance by modulating GDF15 expression to promote embryo implantation; Authors - Guang Yang
human	WB	J Cell Physiol; Tumor-Derived GDF15 Induces Tumor Associated Fibroblast Transformation From BMSCs and Fibroblasts in Oral Squamous Cell Carcinoma; Authors - Jingjing Zhao ; KD Validated
human	WB	Mol Neurobiol; Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation; Authors - Ruicheng Yang
human	WB	Antioxidants (Basel); Grape Seed Proanthocyanidins Induce Apoptosis and Cell Cycle Arrest of HepG2 Cells Accompanied by Induction of the MAPK Pathway and NAG-1.; Authors - Lihua Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anna (Verified Customer) (08-01-2025)

Working nicely for Western Blot applications using a dilution of 1:5000.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Human