GDF-15 Monoklonaler Antikörper

GDF-15 Monoklonal Antikörper für WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

1B1H4

Kat-Nr. : 68768-1-PBS

Synonyme

GDF15, 1B1H4, GDF 15, growth differentiation factor 15, Growth/differentiation factor 15

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  WB analysis using 68768-1-Ig (same clone as 68768-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

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  WB analysis using 68768-1-Ig (same clone as 68768-1-PBS)

  Untreated and Brefeldin A treated LNCaP cells were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

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  WB analysis using 68768-1-Ig (same clone as 68768-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

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  WB analysis of Caki-1 using 68768-1-Ig (same clone as 68768-1-PBS)

  Caki-1 cells were subjected to SDS PAGE followed by western blot with 68768-1-Ig (GDF15 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50133-1

  Sandwich ELISA standard curve of MP50133-1, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-3-PBS. Detection antibody: HRP-conjugated 68768-1-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

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  Sandwich ELISA standard curve of MP50133-2

  Sandwich ELISA standard curve of MP50133-2, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-4-PBS. Detection antibody: HRP-conjugated 68768-1-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

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  Sandwich ELISA standard curve of MP50133-4

  Sandwich ELISA standard curve of MP50133-4, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-7-PBS. Detection antibody: HRP-conjugated 68768-1-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

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  Sandwich ELISA standard curve of MP50133-5

  Sandwich ELISA standard curve of MP50133-5, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-1-PBS. Detection antibody: HRP-conjugated 68768-2-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

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  Sandwich ELISA standard curve of MP50133-8

  Sandwich ELISA standard curve of MP50133-8, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-5-PBS. Detection antibody: HRP-conjugated 68768-1-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

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  Sandwich ELISA standard curve of MP50133-9

  Sandwich ELISA standard curve of MP50133-9, GDF15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-6-PBS. Detection antibody: HRP-conjugated 68768-1-PBS. Standard:Ag33750. Range: 1.563-100 ng/mL.

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  Sample test of MP50133-1

  Cytometric bead array of cell line supernatant using MP50133-1, GDF-15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-3-PBS. Detection antibody: 68768-1-PBS. Supernatant (50μL/well) from the following cell lines were assayed: HepG2, LnCap, HT-1080. Complete culture medium was served as blank control.

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  Sample test of MP50133-2

  Cytometric bead array of cell line supernatant using MP50133-2, GDF-15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-4-PBS. Detection antibody: 68768-1-PBS. Supernatant (50μL/well) from the following cell lines were assayed: HepG2, LnCap, HT-1080. Complete culture medium was served as blank control.

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  Sample test of MP50133-4

  Cytometric bead array of cell line supernatant using MP50133-4, GDF-15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-7-PBS. Detection antibody: 68768-1-PBS. Supernatant (50μL/well) from the following cell lines were assayed: LnCap, HT-1080. Complete culture medium was served as blank control.

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  Sample test of MP50133-5

  Cytometric bead array of cell line supernatant using MP50133-5, GDF-15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-1-PBS. Detection antibody: 68768-2-PBS. Supernatant (50μL/well) from the following cell lines were assayed: LnCap, HT-1080. Complete culture medium was served as blank control.

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  Sample test of MP50133-8

  Cytometric bead array of LnCap cells supernatant using MP50133-8, GDF-15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-5-PBS. Detection antibody: 68768-1-PBS. Complete culture medium was served as blank control.

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  Sample test of MP50133-9

  Cytometric bead array of cell line supernatant using MP50133-9, GDF-15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68768-6-PBS. Detection antibody: 68768-1-PBS. Supernatant (50μL/well) from the following cell lines were assayed: LnCap, HT-1080. Complete culture medium was served as blank control.

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  IHC staining of human placenta using 68768-1-Ig (same clone as 68768-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68768-1-Ig (GDF-15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68768-1-Ig (same clone as 68768-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68768-1-Ig (GDF-15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68768-1-Ig (same clone as 68768-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GDF15 antibody (68768-1-Ig, Clone: 1B1H4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68768-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68768-1-PBS bindet in WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test GDF-15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GDF-15 fusion protein Ag33750
• Vollständiger Name: growth differentiation factor 15
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC008962
• Gene symbol: GDF15
• Gene ID (NCBI): 9518
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Growth differentiation factor 15 (GDF15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily that regulates inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.