GDF8/Myostatin Polyklonaler Antikörper

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• KD/KO Validated

GDF8/Myostatin Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19142-1-PBS

Synonyme

GDF8, MSTN, myostatin, GDF 8, GDF-8

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• 

  WB analysis using 19142-1-AP (same clone as 19142-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 19142-1-AP (same clone as 19142-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 19142-1-AP (same clone as 19142-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 19142-1-AP (same clone as 19142-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 19142-1-AP (same clone as 19142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 19142-1-AP (same clone as 19142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 19142-1-AP (same clone as 19142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19142-1-PBS bindet in WB, IHC, Indirect ELISA GDF8/Myostatin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GDF8/Myostatin fusion protein Ag13605
• Vollständiger Name: myostatin
• Berechnetes Molekulargewicht: 375 aa, 43 kDa
• Beobachtetes Molekulargewicht: 50 and 35 kDa
• GenBank-Zugangsnummer: BC074757
• Gene symbol: MSTN
• Gene ID (NCBI): 2660
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myostatin, also known as GDF-8, is a member of the TGF-beta superfamily. Myostatin is expressed in the myotome and developing skeletal muscles. Myostatin negatively regulates skeletal muscle growth and development through the regulation of anabolic and catabolic pathways in skeletal muscles. Myostatin immunoreactive bands could be identified at ∼50, 32-35, and 16 kDa, which correspond to the precursor, glycosylated dimmer, and monomer, respectively (PMID: 17711997). GDF8 may have cross-reactions with GDF11.