GDF9 Polyklonaler Antikörper

GDF9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 29309-1-AP

Synonyme

GDF-9, GDF 9

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  WB analysis using 29309-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29309-1-AP (GDF9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 29309-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 29309-1-AP (GDF9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SKOV-3 using 29309-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using GDF9 antibody (29309-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, Maus-Eierstockgewebe, Ratten-Eierstockgewebe, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: Maus-Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

29309-1-AP bindet in WB, IHC, IF/ICC, ELISA GDF9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GDF9 fusion protein Ag29603
• Vollständiger Name: growth differentiation factor 9
• Berechnetes Molekulargewicht: 454 aa, 51 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC096228
• Gene symbol: GDF9
• Gene ID (NCBI): 2661
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GDF9 (Growth/differentiation factor 9) belongs to the TGF-beta family. GDF9 is required for ovarian folliculogenesis. GDF9 and BMP15 are oocyte-secreted factors with a leading role in the control of ovarian function in female reproduction, modulating both the cell fate of the somatic granulosa cells and the quality and developmental competence of the egg (PMID: 29544636). GDF9 promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation (PMID: 19366876). It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. GDF9 attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B (PMID: 21829661). It suppresses FST and FSTL3 production in granulosa-lutein cells  (PMID: 21632818, PMID: 21829661).

Publikationen

Species	Application	Title
mouse	IF	Apoptosis; CCDC134 enhances ovarian reserve function and angiogenesis by directly interacting with INHA in a mouse model of premature ovarian insufficiency; Authors - Xiangrong Cui

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yiran (Verified Customer) (09-19-2024)

Works well on paraffin-fixed sheep ovary for immunofluorescent

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Ovary

GDF9 Antibody Immunohistochemistry validation (1:100 dilution) in Ovary (Cat no:29309-1-AP)