GDI1 Polyklonaler Antikörper

GDI1 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55214-1-PBS

Synonyme

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  WB analysis of SH-SY5Y using 55214-1-AP (same clone as 55214-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55214-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 55214-1-AP (same clone as 55214-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55214-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 55214-1-AP (same clone as 55214-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55214-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 55214-1-AP (same clone as 55214-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55214-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 55214-1-AP (same clone as 55214-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55214-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 55214-1-AP (same clone as 55214-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55214-1-AP (GDI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55214-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55214-1-PBS bindet in WB, Indirect ELISA GDI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GDP dissociation inhibitor 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 51-55 kDa
• GenBank-Zugangsnummer: NM_001493
• Gene symbol: GDI1
• Gene ID (NCBI): 2664
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55kda protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034). Defects in GDI1 are the cause of mental retardation X-linked type 41 (MRX41). Defects in GDI1 are the cause of mental retardation X-linked type 48 (MRX48). The antibody is specific to GDI1. And it has no cross reaction to GDI2.