GDI1 Monoklonaler Antikörper

GDI1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4E1G1

Kat-Nr. : 66434-1-PBS

Synonyme

OPHN2, RABGDIA, XAP4, MRX41, GDP dissociation inhibitor 1

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  WB analysis using 66434-1-Ig (same clone as 66434-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66434-1-Ig (same clone as 66434-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 66434-1-Ig (same clone as 66434-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66434-1-Ig (same clone as 66434-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66434-1-Ig (same clone as 66434-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66434-1-Ig (same clone as 66434-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66434-1-Ig (same clone as 66434-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66434-1-Ig (GDI1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66434-1-Ig (same clone as 66434-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66434-1-Ig (GDI1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66434-1-Ig (same clone as 66434-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66434-1-Ig (GDI1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66434-1-Ig (same clone as 66434-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66434-1-Ig (GDI1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66434-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66434-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GDI1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GDI1 fusion protein Ag17511
• Vollständiger Name: GDP dissociation inhibitor 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC000317
• Gene symbol: GDI1
• Gene ID (NCBI): 2664
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55 kDa protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034)