GDI2 Polyklonaler Antikörper

GDI2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Rezensionen (1)

Kat-Nr. : 10116-1-AP

Synonyme

GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, Rab GDI β, RABGDIB

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Galerie der Validierungsdaten

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  WB analysis of A431 using 10116-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10116-1-AP (GDI2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10116-1-AP (GDI2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 10116-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10116-1-AP (GDI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10116-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10116-1-AP (GDI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10116-1-AP (GDI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10116-1-AP (GDI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, Maushirngewebe, Mausmilzgewebe, Rattenhirngewebe, Rattenmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

10116-1-AP bindet in WB, IHC, IF, ELISA GDI2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hefe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GDI2 fusion protein Ag0170
• Vollständiger Name: GDP dissociation inhibitor 2
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC005145
• Gene symbol: GDI2
• Gene ID (NCBI): 2665
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.

Publikationen

Species	Application	Title
monkey	WB	Science; A bifunctional bacterial protein links GDI displacement to Rab1 activation.; Authors - Machner Matthias P MP
yeast	WB	Proc Natl Acad Sci U S A; Structural mechanism of host Rab1 activation by the bifunctional Legionella type IV effector SidM/DrrA.; Authors - Zhu Yongqun Y
mouse	IF	Curr Biol; Rab34 GTPase mediates ciliary membrane formation in the intracellular ciliogenesis pathway.; Authors - Anil Kumar Ganga
human	WB	Hum Mol Genet; Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.; Authors - McCray Brett A BA
mouse	WB	Mol Cell Proteomics; Differential proteomics analysis of synaptic proteins identifies potential cellular targets and protein mediators of synaptic neuroprotection conferred by the slow Wallerian degeneration (Wlds) gene.; Authors - Wishart Thomas M TM
human	IHC	Endocr Relat Cancer; Comprehensive gene expression profiling of anaplastic thyroid cancers with cDNA microarray of 25 344 genes.; Authors - Onda M M

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guo-rong (Verified Customer) (08-30-2022)

Specific and clear results

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: ARPE-19

GDI2 Antibody Western Blot validation (1:2000 dilution) in ARPE-19 (Cat no:10116-1-AP)