GDPD1 Polyklonaler Antikörper

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• KD/KO Validated

GDPD1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 27861-1-AP

Synonyme

EC:3.1.4.-, GDE4, Glycerophosphodiester phosphodiesterase domain-containing protein 1, Lysophospholipase D GDPD1, UGPQ

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  WB analysis using 27861-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27861-1-AP (GDPD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 27861-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27861-1-AP (GDPD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 27861-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27861-1-AP (GDPD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 27861-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27861-1-AP (GDPD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 27861-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27861-1-AP (GDPD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Maus-Eierstockgewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

27861-1-AP bindet in WB, IHC, ELISA GDPD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GDPD1 fusion protein Ag27371
• Vollständiger Name: glycerophosphodiester phosphodiesterase domain containing 1
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 33-36 kDa
• GenBank-Zugangsnummer: BC034432
• Gene symbol: GDPD1
• Gene ID (NCBI): 284161
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycerophosphodiester phosphodiesterase domain-containing protein 1 (GDPD1) is an enzyme that hydrolyzes lysoglycerophospholipids to produce lysophosphatidic acid (LPA) and the corresponding amines. GDPD1 shows a preference for 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF), lysophosphatidylethanolamine (lyso-PE) and lysophosphatidylcholine (lyso-PC). GDPD1 may be involved in bioactive N-acylethanolamine biosynthesis from both N-acyl-lysoplasmenylethanolamin (N-acyl-lysoPlsEt) and N-acyl-lysophosphatidylethanolamin (N-acyl-lysoPE) (PMID:25596343, PMID:27637550). GDPD1 is widely expressed with a high expression level in testis. GDPD1 is a prognostic marker in Liver hepatocellular carcinoma.

Publikationen

Species	Application	Title
human	WB,IHC	Cell Death Differ; A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA.; Authors - Xiaoyu Qi
human	WB	J Lipid Res; Development of a selective fluorescence-based enzyme assay for glycerophosphodiesterase family members GDE4 and GDE7; Authors - Keisuke Kitakaze ; KO Validated