GEMIN5 Polyklonaler Antikörper

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• KD/KO Validated

GEMIN5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24897-1-PBS

Synonyme

Gem associated protein 5, Gem-associated protein 5

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  WB analysis using 24897-1-AP (same clone as 24897-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24897-1-AP (GEMIN5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 24897-1-AP (same clone as 24897-1-PBS)

  WB result of GEMIN5 antibody (24897-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GEMIN5 transfected HEK-293 cells. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 24897-1-AP (same clone as 24897-1-PBS)

  IP result of anti-GEMIN5 (IP:24897-1-AP, 4ug; Detection:24897-1-AP 1:1000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 24897-1-AP (same clone as 24897-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24897-1-AP (GEMIN5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 24897-1-AP (same clone as 24897-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24897-1-AP (GEMIN5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 24897-1-AP (same clone as 24897-1-PBS)

  Immunofluorescent analysis of HeLa cells using 24897-1-AP (GEMIN5 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24897-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA GEMIN5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GEMIN5 fusion protein Ag19268
• Vollständiger Name: gem (nuclear organelle) associated protein 5
• Berechnetes Molekulargewicht: 1508 aa, 169 kDa
• Beobachtetes Molekulargewicht: 168 kDa
• GenBank-Zugangsnummer: BC113614
• Gene symbol: GEMIN5
• Gene ID (NCBI): 25929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.