GEMIN5 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

GEMIN5 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241205D8

Kat-Nr. : 84171-3-RR

Synonyme

241205D8, Gem associated protein 5, Gem-associated protein 5

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  WB analysis of HEK-293 using 84171-3-RR

  WB result of GEMIN5 antibody (84171-3-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GEMIN5 transfected HEK-293 cells.

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  WB analysis using 84171-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84171-3-RR (GEMIN5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84171-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GEMIN5 antibody (84171-3-RR, Clone: 241205D8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84171-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84171-3-RR against Human GEMIN5 were performed. The affinity constant is 0.562 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84171-3-RR bindet in WB, IF/ICC, ELISA GEMIN5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GEMIN5 fusion protein Ag19220
• Vollständiger Name: gem (nuclear organelle) associated protein 5
• Berechnetes Molekulargewicht: 1508 aa, 169 kDa
• GenBank-Zugangsnummer: BC113614
• Gene symbol: GEMIN5
• Gene ID (NCBI): 25929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.